Selected Publications
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Locomotion activates PKA through dopamine and adenosine in striatal neurons
Lei Ma, Julian Day-Cooney, Omar Jáidar Benavides, Michael A. Muniak, Maozhen Qin, Jun B. Ding, Tianyi Mao & Haining Zhong. Nature (2022). https://doi.org/10.1038/s41586-022-05407-4
“Our results suggest that acute adenosine accumulation interplays with dopamine release to orchestrate protein kinase A (PKA) activity in striatal spiny projection neurons (SPNs) and proper striatal function during animal locomotion.”
Check out the full article in Nature here.
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Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo
Crystian I. Massengill, Landon Bayless-Edwards, Cesar C. Ceballos, Elizabeth R. Cebul, James Cahill, Arpita Bharadwaj, Evan Wilson, Maozhen Qin, Matthew R. Whorton, Isabelle Baconguis, Bing Ye, Tianyi Mao & Haining Zhong (2022) Nat Methods. https://doi.org/10.1038/s41592-022-01646-5
Published in Nature Methods, the Zhong Lab and collaborators have developed a genetically encoded cAMP sensor, cleverly dubbed “cAMPFIRE”. This sensor can detect nanomolar concentrations of cAMP, can be used in vivo, and is compatible with both ratiometric and fluorescence lifetime imaging. The cAMPFIRE sensor will be a valuable tool for the scientific community, and we couldn’t be more excited about it.
Check out the paper here.
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Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE)
Wilson, E., Mao, T. and Zhong, H. (2022) Bio-protocol.
This published bio-protocol details the background, materials, and protocol for performing the CRISPIE genome editing methodology developed in Zhong et al. 2021. Check out the protocol here. Bio-protocol is a journal that publishes detailed protocols from previously published papers, with the aim of improving reproducibility and transparency in science.
Genetically encoded sensors towards imaging cAMP and PKA activity in vivo
Crystian I Massengill, Julian Day-Cooney, Tianyi Mao, Haining Zhong (2021). J Neurosci Methods.
This review summarizes genetically encoded cAMP and PKA sensors for in vivo imaging.